Part:BBa_K4017001:Design
CAP Biding site
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is composed of the site for the catabolite activator protein to bind. The Escherichia coli catabolite activator protein activates transcription for the lac promoter (BBa_M36411) by binding to its specific DNA sites in the presence of cAMP and thus enhancing the ability of RNA polymerase to bind and initiate transcription [1]. Promoter activity is also influenced by glucose levels: in its absence cAMP levels are high and the complex cAMP+CAP binds to lac promoter, beginning transcription. In the presence of glucose, cAMP levels are low and promoter activity is inhibited. It is then the simplest but effective transcription activation in bacteria cultures like E. coli and B. subtilis [1].
Source
Adapted from the genomic sequence of Escherichia coli DH5α.
References
[1]: Lawson, Catherine L., et al. "Catabolite activator protein: DNA binding and transcription activation." Current opinion in structural biology 14.1 (2004): 10-20.